Optical microscopy that visualizes microscopic worlds beyond human vision has revolutionized biology and other branches of science. It also plays a central role nowadays in many engineering disciplines and manufacturing industry. Yet, development of optical microscopy methods is still an active field of research in response to ever-increasing demands on new imaging capabilities such as super-resolution, deep penetration, and fast volumetric imaging. This seminar covers a recent advance in super-resolution microscopy for deep-tissue investigation. Starting from an introduction to optical microscopy for biology, I will explain single-molecule localization microscopy, a super-resolution imaging method that offers nanoscale technique applicable to higher-level samples like tissues and small animals, I will present a new single-molecule microscopy platform (named obSTORM) based on oblique light-sheet illumination and oblique fluorescence detection. obSTORM features oblique, multicolor, and volumetric super-resolution imaging deep inside thick samples. obSTORM images of diverse biological samples from cells to small animals will be explored.